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National Public Health Institute
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articles, bacillus subtilis, publications, research

Julkaistu 12.5.2008

Bacillus subtilis on ei-patogeeninen, Gram-positiivinen bakteerimalli. Hyödynnämme sen helppoa käsiteltävyyttä ja olemassa olevaa laajaa tietämystä sen genetiikasta ja fysiologiasta tutkiessamme bakteerisolujen molekylaarisia perusmekanismeja. Tutkimuskohteemme liittyvät signaalivälitykseen, stressimekanismeihin, soluseinämän muodostumiseen ja proteiinieritykseen.

PrsA-lipoproteiini katalysoi erittyvien proteiinien oikean rakenteen muodostumista gram-positiivisen bakteerin soluseinämässä. Tätä ominaisuutta voidaan hyödyntää biotekniikassa eräiden proteiinien tuotannon lisäämisessä.
 

Keskeiset julkaisut:

1. Heinrich, J., Lundén, T., Kontinen, V.P. and Wiegert, T. The Bacillus subtilis ABC transporter EcsAB influences intramembrane proteolysis through RasP. Microbiology, in press.

2. Sood, R., Domanov, Y., Pietiäinen, M., Kontinen, V.P. and Kinnunen, P.K.J. (2008) Binding of LL-37 to model biomembranes: Insight into targets vs host cell recognition. Biochim Biophys Acta 1778, 983-996.

3. Hyyryläinen, H-L., Pietiäinen, M., Lunden, T., Ekman, A., Gardemeister, M., Antelmann, H., Hecker, M., Valmu, L., Sarvas, M. and Kontinen, V.P. (2007) The density of negative charge in the cell wall influences two-component signal transduction in Bacillus subtilis. Microbiology 153, 2126-2136.

4. Pietiäinen, M., Gardemeister, M., Mecklin, M., Leskelä, S., Sarvas, M. and Kontinen V.P. (2005) Cationic antimicrobial peptides elicit a complex stress response in Bacillus subtilis that involves ECF-type sigma factors and two-component signal transduction systems. Microbiology 151, 1577-1592.

5. Hyyryläinen, H-L., Sarvas, M. and Kontinen, V.P. (2005) Transcriptome analysis of the secretion stress response of Bacillus subtilis: htrA, htrB, liaIHG and yqxL genes exhibit the strongest induction patterns. Appl Miocrobiol Biot 67, 389-396.

6. Vitikainen, M., Hyyryläinen, H-L., Kivimäki, A., Kontinen, V.P. and Sarvas M. (2005) Secretion of heterologous proteins in Bacillus subtiliscan be improved by engineering cell components affecting posttranslocational protein folding and degradation. J Appl Microbiol, 99, 363-375.

7. Vitikainen, M., Lappalainen, I., Seppala, R., Antelmann, H., Boer, H., Taira, S., Savilahti, H., Hecker, M., Vihinen, M., Sarvas, M. and Kontinen, V.P. (2004) Structure-function analysis of PrsA reveals roles for the parvulin-like and flanking N- and C-terminal domains in protein folding and secretion in Bacillus subtilis. J Biol Chem 279, 19302-19314.

8. Craynest, M., Joergensen, S., Sarvas, M., Kontinen, V.P. (2003) Enhanced production of cyclodextrin glycosyltransferase by a mutant of Bacillus licheniformis defective in the D-alanylation of teichoic acids. Lett Appl Microbiol 37, 75-80.

9. Wahlström, E., Vitikainen, M., Kontinen, V.P. and Sarvas, M. (2003) The extracytoplasmic folding factor PrsA is required for protein secretion only in the presence of the cell wall in Bacillus subtilis. Microbiology 149, 569-577.

10. Pummi, T., Leskelä, S., Wahlström, E., Gerth, U., Tjalsma, H., Hecker, M., Sarvas, M. and Kontinen, V.P. (2002) ClpXP protease regulates the signal peptide cleavage of secretory preproteins in Bacillus subtilis with a mechanism distinct from that of the Ecs ABC transporter. J Bact 184, 1010-1018.

11. Vitikainen, M. Pummi, T., Airaksinen, U., Wu, H., Sarvas, M. and Kontinen, V.P. (2001) Quantitation of the capacity of the secretion apparatus and requirement for PrsA in the growth and secretion of alpha-amylase in Bacillus subtilis. J Bact 183, 1881-1890.

12. Hyyryläinen, H-L., Bolhuis, A., Darmon, E., Vitikainen, M., Muukkonen, L.,  Koski, P., Sarvas, M., Prágai, Z., Bron, S., van Dijl, J.M., and Kontinen, V.P. (2001) A novel two-component regulatory system of Bacillus subtilis for the survival of severe secretion stress. Mol Microbiol 41, 1159-1172.

13. Hyyryläinen, H-L., Vitikainen, M., Thwaite, J., Wu, H., Harwood, C., Sarvas, M., Kontinen, V.P. and Stephenson, K. (2000) D-alanine substitution of teichoic acids as a modulator of protein folding and stability at the cytoplasmic membrane-cell wall interface of Bacillus subtilis. J Biol Chem 275, 26696-26703.

14. Leskelä, S., Wahlström, E., Hyyryläinen, H.-L., Jacobs, M., Palva, A., Sarvas, M. and Kontinen, V.P. (1999) Ecs, an ABC transporter of Bacillus subtilis: dual signal transduction functions affecting expression of secreted proteins as well as their secretion. Mol Microbiol 31, 533-544.

15. Leskelä, S., Wahlström, E., Kontinen, V.P. and Sarvas, M. (1999) Lipid modification of prelipoproteins is dispensible for growth but essential for efficient protein secretion in Bacillus subtilis: characterization of the lgt gene. Mol Microbiol 31, 1075-1087.

16. Tjalsma, H., Kontinen, V.P., Pragai, Z., Wu, H., Meima, R.B., Venema, G., G., Bron, S., Sarvas, M., and van Dijl, J.M. (1999) The role of lipoprotein processing by signal peptidase II in the gram-positive eubacterium Bacillus subtilis. Signal peptidase II is required for efficient secretion of alpha-amylase, a non-lipoprotein. J Biol Chem 274, 1698-1707.

17. Bolhuis, A., Matzen, A., Hyyryläinen, H-L., Kontinen, V.P., Meima R., Chapuis, J., Venema, G., Bron, S., Freudl, R., and van Dijl, J.M. (1999) Signal peptide peptidase- and ClpP-like proteins of Bacillus subtilis required for efficient translocation and processing of secretory proteins. J Biol Chem 274, 24585-24592.

18. Leskelä, S., Kontinen, V. and Sarvas, M. (1996) Molecular analysis of an operon in Bacillus subtilis encoding a novel ABC transporter with a role in exoprotein production, sporulation and competence. Microbiology 142, 71-77.

19. Kontinen, V.P. and Sarvas, M. (1993) The PrsA lipoprotein is essential for protein secretion in Bacillus subtilis and sets a limit for high level secretion. Mol Microbiol 8, 727-737.

20. Jacobs, M., Andersen, J.B., Kontinen, V.P. and Sarvas, M. (1993) Bacillus subtilis PrsA is required in vivoas an extracytoplasmic chaperone for secretion of active enzyme synthesized either with or without pro-sequences. Mol Microbiol 8, 957-966.

21. Kontinen, V.P., Saris, P. and Sarvas, M. (1991) A gene (prsA) of Bacillus subtilis involved in a novel, late step of protein export. Mol Microbiol 5, 1273-1283.

22. Kontinen, V.P. and Sarvas, M. (1988) Mutants of Bacillus subtilis defective in protein export. J Gen Microbiol 134, 2333-2344.